Localization of p53, Retinoblastoma and Host Replication Proteins at Sites of Viral Replication in Herpes-Infected Cells

Nature. 1991 Jan 31;349(6308):429-31. doi: 10.1038/349429a0.

Abstract

Replication of DNA occurs at discrete sites in eukaryotic cell nuclei, where replication proteins are clustered into large complexes, or 'replicases'. Similarly, viral DNA replication is a highly structured process, notably in herpes simplex virus type-1 (HSV-1; reviewed in ref. 4) in which large globular 'replication compartments' containing the viral replication machinery exist. Replicating cellular DNA redistributes to these compartments upon HSV-1 infection. We have now used antibodies raised against several cellular proteins to detect changes in their subnuclear localization on HSV-1 infection. We found that various proteins involved in cellular DNA replication move to sites of viral DNA synthesis, whereas a selection of non-replication proteins do not. The retinoblastoma protein and p53 (the products of two putative anti-oncogenes) relocate to the same sites as known DNA replication proteins, suggesting that they may be associated with DNA replication complexes in normal, uninfected cells.

MeSH terms

  • Animals
  • Cell Compartmentation
  • DNA Replication*
  • DNA-Binding Proteins / metabolism
  • Fluorescent Antibody Technique
  • Humans
  • Nuclear Proteins / metabolism
  • Phosphonoacetic Acid / pharmacology
  • Proliferating Cell Nuclear Antigen
  • Retinoblastoma Protein / metabolism*
  • Simplexvirus / metabolism*
  • Tumor Suppressor Protein p53 / metabolism*
  • Viral Proteins / metabolism
  • Virus Replication*

Substances

  • DNA-Binding Proteins
  • ICP8 protein, Simplexvirus
  • Nuclear Proteins
  • Proliferating Cell Nuclear Antigen
  • Retinoblastoma Protein
  • Tumor Suppressor Protein p53
  • Viral Proteins
  • Phosphonoacetic Acid