Expression of Kaposi's sarcoma-associated herpesvirus-encoded K10/10.1 protein in tissues and its interaction with poly(A)-binding protein

Virology. 2006 Aug 15;352(1):100-9. doi: 10.1016/j.virol.2006.04.009. Epub 2006 May 22.

Abstract

The K10/10.1 protein is encoded by a cluster of interferon regulatory factor (IRF) homologues in the Kaposi's sarcoma-associated herpesvirus (KSHV, human herpesvirus 8, HHV-8) genome. In the present study, we showed that an anti-K10 antibody reacted with a 110-kDa protein encoded by the K10/10.1 gene of KSHV in KSHV-infected primary effusion lymphoma (PEL) cell lines. Expression of K10/10.1 protein was induced by phorbol ester in KSHV-infected cells. A reporter gene assay demonstrated that K10/10.1 protein did not influence promoter activity of human interferon genes, regardless of its homology to human IRFs. Poly(A)-binding protein (PABP) was identified as a partner of K10/10.1 protein. Immunoprecipitation revealed that K10/10.1 protein interacted with PABP specifically in PEL cell lines. IFA revealed co-localization of K10/10.1 protein and PABP in the nucleus of KSHV-infected cells. These data suggest that K10/10.1 protein may affect the translational status or stability of mRNA in host cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Avian Proteins / chemistry
  • Avian Proteins / genetics
  • Avian Proteins / metabolism*
  • Cell Line
  • Herpesvirus 8, Human / metabolism
  • Herpesvirus 8, Human / pathogenicity*
  • Humans
  • Interferon Regulatory Factors / chemistry
  • Interferon Regulatory Factors / genetics
  • Interferon Regulatory Factors / metabolism*
  • Molecular Sequence Data
  • Poly A / metabolism
  • Poly(A)-Binding Proteins / metabolism*

Substances

  • Avian Proteins
  • Interferon Regulatory Factors
  • Poly(A)-Binding Proteins
  • interferon regulatory factor-10, Gallus gallus
  • Poly A