Abstract
The work presented herein is a new noncovalent glycoarray assembly method for microplates created by simply mixing together a carbohydrate and a tetradecylamine. alpha-D-Mannopyranoside, alpha-D-glucopyranoside, and alpha-D-galactopyranoside were utilized in model studies and product formations were detected by lectin binding. The method can be extended to study the steric hindrance effect of carbohydrate-protein interactions, namely the structure-function relations of carbohydrates.
MeSH terms
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Amines / chemistry
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Binding Sites
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Carbohydrates / chemistry*
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Concanavalin A / chemistry
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Concanavalin A / metabolism
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Fluorescein-5-isothiocyanate / chemistry
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Galactose / chemistry
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Glucosides / chemistry
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Glycolipids / chemistry*
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Glycolipids / metabolism
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Lectins / chemistry
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Lectins / metabolism
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Mannose / chemistry
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Microscopy, Fluorescence
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Models, Chemical
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Proteins / chemistry
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Proteins / metabolism
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Structure-Activity Relationship
Substances
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Amines
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Carbohydrates
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Glucosides
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Glycolipids
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Lectins
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Proteins
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Concanavalin A
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Fluorescein-5-isothiocyanate
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N-tetradecylamine
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Mannose
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Galactose