CDC2L5, a Cdk-like kinase with RS domain, interacts with the ASF/SF2-associated protein p32 and affects splicing in vivo

J Cell Biochem. 2006 Oct 15;99(3):890-904. doi: 10.1002/jcb.20986.


The human CDC2L5 gene encodes a protein of unknown physiological function. This protein is closely related to the cyclin-dependent kinase (Cdks) family and contains an arginine/serine-rich (RS) domain. The Cdks were first identified as crucial regulators of cell-cycle progression, more recently they were found to be involved in transcription and mRNA processing. RS domains are mainly present in proteins regulating pre-mRNA splicing, suggesting CDC2L5 having a possible role in this process. In this study, we demonstrate that CDC2L5 is located in the nucleoplasm, at a higher concentration in speckles, the storage sites for splicing factors. Furthermore, this localization is dependent on the presence of the N-terminal sequence including the RS domain. Then, we report that CDC2L5 directly interacts with the ASF/SF2-associated protein p32, a protein involved in splicing regulation. Overexpression of CDC2L5 constructs disturbs constitutive splicing and switches alternative splice site selection in vivo. These results argue in favor of a functional role of the CDC2L5 kinase in splicing regulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CDC2 Protein Kinase / genetics
  • CDC2 Protein Kinase / metabolism*
  • Cell Line
  • Cell Nucleus / metabolism
  • Cell Nucleus / ultrastructure
  • Humans
  • Lymphotoxin-alpha / genetics
  • Lymphotoxin-alpha / metabolism
  • Mice
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Protein Structure, Tertiary
  • Protein Subunits / genetics
  • Protein Subunits / metabolism*
  • RNA Precursors / metabolism*
  • RNA Splicing*
  • RNA-Binding Proteins
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Serine-Arginine Splicing Factors
  • Two-Hybrid System Techniques


  • Lymphotoxin-alpha
  • Nuclear Proteins
  • Protein Subunits
  • RNA Precursors
  • RNA-Binding Proteins
  • Recombinant Proteins
  • Serine-Arginine Splicing Factors
  • CDC2 Protein Kinase
  • CDK13 protein, human