A liquid chromatography-electrospray ionization tandem mass spectrometric assay for quantitation of the histone deacetylase inhibitor, vorinostat (suberoylanilide hydroxamicacid, SAHA), and its metabolites in human serum

J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Aug 18;840(2):108-15. doi: 10.1016/j.jchromb.2006.04.044. Epub 2006 May 24.


Vorinostat (suberoylanilide hydroxamic acid, SAHA) is undergoing evaluation as an antineoplastic agent. We developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for quantitating vorinostat and its major metabolites, vorinostat glucuronide and 4-anilino-4-oxobutanoic acid, in human serum. The assay uses: deuterated internal standards; acetonitrile protein precipitation; a BDS Hypersil C18 (3 microm, 100 mm x 3 mm) column; a gradient mobile phase of 0.5% acetic acid in acetonitrile and water; and electrospray positive-mode ionization with selected reaction monitoring (SRM) detection. The lower limit of quantitation was 3.0 ng/ml for each analyte. The assay is being employed in at least 12 clinical studies of vorinostat-containing regimens.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Chromatography, Liquid / methods*
  • Enzyme Inhibitors / blood*
  • Histone Deacetylase Inhibitors*
  • Humans
  • Hydroxamic Acids / blood*
  • Reproducibility of Results
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Vorinostat


  • Enzyme Inhibitors
  • Histone Deacetylase Inhibitors
  • Hydroxamic Acids
  • Vorinostat