Biased expression of individual T cell receptor V gene segments in CD4+ and CD8+ human peripheral blood T lymphocytes

Eur J Immunol. 1991 Mar;21(3):819-22. doi: 10.1002/eji.1830210342.


The expression of seven different alpha and beta gene segments of the T cell receptor on normal human CD4+ and CD8+ T lymphocytes, respectively, was examined using V gene-specific monoclonal antibodies. We found a statistically significant (p less than 0.001) bias of the expression of four V gene products towards the CD4+ subpopulation. In every individual analyzed, the V beta 5.1 gene segment was expressed to a higher degree among CD4+ compared to CD8+ cells, with a median value of 4.8% among CD4+ cells and 1.5% among CD8+ cells. There was also a statistically significant skewness in the usage of the V beta 6.7, V beta 8 and V beta 12 gene segments towards the CD4+ T cell population, but not as dominating as for the V beta 5.1. Lymphocytes from umbilical cord blood showed similar skewed reactivities for the V beta 5.1, V beta 6.7 and V beta 12-specific monoclonal antibodies. Aspects of positive and negative selection, as possible explanations for these findings, are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antigens, Differentiation, T-Lymphocyte / analysis
  • CD4-Positive T-Lymphocytes / immunology
  • CD8 Antigens
  • Flow Cytometry
  • Humans
  • Receptors, Antigen, T-Cell / classification
  • Receptors, Antigen, T-Cell / immunology*
  • T-Lymphocyte Subsets / immunology*


  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte
  • CD8 Antigens
  • Receptors, Antigen, T-Cell