Urovirulence determinants in Escherichia coli isolates causing first-episode and recurrent cystitis in women

J Infect Dis. 1991 Apr;163(4):773-9. doi: 10.1093/infdis/163.4.773.

Abstract

To assess the prevalence of urovirulence determinants among Escherichia coli isolates from women with acute uncomplicated cystitis, 121 isolates from 87 women with first-episode or recurrent cystitis and 156 fecal isolates from 52 women without recent urinary tract infection were tested using DNA probes for P fimbriae, hemolysin, aerobactin, and diffuse adhesin and for expression of hemolysin and P and F adhesins. P fimbrial genotype (P = .002), hemolysin phenotype (P = .007), and the diffuse adhesin determinant (P = .03), but not aerobactin, were found more frequently in E. coli from women with acute cystitis, and expression of the F adhesin (41%) was more common than the P adhesin (24%; P = .001). E. coli isolates that caused cystitis in women using diaphragms had fewer virulence determinants than those from nonusers (P = .04), suggesting that diaphragm use may allow infection with less virulent E. coli.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acute Disease
  • Adhesins, Escherichia coli
  • Adult
  • Bacterial Adhesion
  • Bacterial Outer Membrane Proteins / analysis
  • Cystitis / microbiology*
  • DNA Probes
  • Escherichia coli / pathogenicity*
  • Escherichia coli Infections / microbiology*
  • Female
  • Fimbriae, Bacterial
  • Genotype
  • Hemolysin Proteins / analysis
  • Humans
  • Hydroxamic Acids / analysis
  • Nucleic Acid Hybridization
  • Phenotype
  • Recurrence
  • Virulence

Substances

  • Adhesins, Escherichia coli
  • Bacterial Outer Membrane Proteins
  • DNA Probes
  • Hemolysin Proteins
  • Hydroxamic Acids
  • aerobactin