A Novel Recombinant Virus-Like Particle Vaccine for Prevention of Porcine Parvovirus-Induced Reproductive Failure

Vaccine. 2006 Jun 29;24(26):5481-90. doi: 10.1016/j.vaccine.2006.03.089. Epub 2006 Apr 18.

Abstract

A novel vaccine against porcine parvovirus (PPV), composed of recombinant virus-like particles (PPV-VLPs) produced with the baculovirus expression vector system (BEVS) at industrial scale, was tested for its immunogenicity and protective potency. A formulation of submicrogram amounts of PPV-VLPs in a water-in-mineral oil adjuvant evoked high serum antibody titres in both guinea pigs, used as reference model, and target species, pigs. A single immunisation with 0.7microg of this antigen yielded complete foetal protection against PPV infection after challenge with a virulent strain of this virus. Furthermore, also in the presence of mild adjuvants the protective action of these PPV-VLPs is excellent. This recombinant subunit vaccine overcomes some of the drawbacks of classical PPV vaccines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic
  • Animals
  • Dose-Response Relationship, Drug
  • Female
  • Guinea Pigs
  • Parvoviridae Infections / prevention & control
  • Parvoviridae Infections / veterinary*
  • Parvovirus, Porcine / immunology*
  • Pregnancy
  • Swine
  • Swine Diseases / prevention & control*
  • Viral Vaccines / adverse effects
  • Viral Vaccines / immunology*

Substances

  • Adjuvants, Immunologic
  • Viral Vaccines