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, 134 (6), 991-6

Biofilm Formation by Staphylococcus Aureus and Pseudomonas Aeruginosa Is Associated With an Unfavorable Evolution After Surgery for Chronic Sinusitis and Nasal Polyposis

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Biofilm Formation by Staphylococcus Aureus and Pseudomonas Aeruginosa Is Associated With an Unfavorable Evolution After Surgery for Chronic Sinusitis and Nasal Polyposis

Zohra Bendouah et al. Otolaryngol Head Neck Surg.

Abstract

Objectives: To determine whether biofilm-forming capacity of bacteria demonstrated in chronic rhinosinusitis (CRS) has an impact on persistence of the disease following endoscopic sinus surgery (ESS).

Method: Thirty-one bacterial strains recovered from 19 patients with CRS at least 1 year post-ESS. Evolution of disease was assessed by questionnaire and endoscopy as favorable or unfavorable. The bacteria were cultured on a 96-well culture plaque and a semi-quantitative method using crystal violet to quantify biofilm production was used.

Results: Twenty-two of 31 samples produced a biofilm thicker or equal to the positive control. Biofilm production was noted in 6/10 Pseudomonas aeruginosa isolates, 8/10 Staphylococcus aureus, and 8/11 coagulase-negative staphylococci. Biofilm formation was associated with a poor evolution for Pseudomonas aeruginosa and Staphylococcus aureus, but not coagulase-negative staphylococcus.

Conclusion: There is a correlation between in vitro biofilm-producing capacity by Pseudomonas aeruginosa and Staphylococcus aureus and unfavorable evolution after ESS, suggesting a role for biofilm production in chronic sinusitis.

Ebm rating: B-2b.

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