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. 2006 May;14(5):346-9.

[Screening the hALR-interacting Protein From the cDNA Library of Hepatocarcinoma Cells and Studying Its Biological Functions]

[Article in Chinese]
Affiliations
  • PMID: 16732908

[Screening the hALR-interacting Protein From the cDNA Library of Hepatocarcinoma Cells and Studying Its Biological Functions]

[Article in Chinese]
Jian-chuan Deng et al. Zhonghua Gan Zang Bing Za Zhi. .

Abstract

Objectives: To screen the hALR-interacting protein by phage-displayed technique and identify its biological activities.

Methods: The specific phage clones that interacted with target protein hALR from a cDNA library of hepatocarcinoma cells were selected using the T7 phage-displayed technique. The acquired cDNA inserts were sequenced and analyzed by bioinformatic tools. The biological activities of the phage-displayed peptide affecting QGY hepatocarcinoma cells were studied using 3H-TdR method.

Results: The cDNA inserts with 212 bp were acquired after 4 rounds of biopanning. They showed 100% homology with citron kinase. The phage-displayed peptide and the peptide combined with hALR affected QGY cells proliferation.

Conclusions: hALR-interacting peptide can be specifically screened by phage-displayed technique. Citron kinase that interacted with hALR potentially plays an important role in the proliferation of hepatocarcinoma cells.

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