Molecular cloning and chromosomal localization of a novel human tracheo-bronchial mucin cDNA containing tandemly repeated sequences of 48 base pairs

Biochem Biophys Res Commun. 1991 Mar 15;175(2):414-22. doi: 10.1016/0006-291x(91)91580-6.


A lambda gt11 cDNA library constructed from human tracheo-bronchial mucosa was screened with a polyclonal antiserum raised to chemically deglycosylated pronase glycopeptides from human bronchial mucins. Out of 20 positives clones, one partial cDNA clone was isolated and allowed to map a novel human tracheo-bronchial mucin gene. It contains 48 nucleotide tandem repeats quite perfectly identical which encodes a protein containing about 50% of hydroxy amino-acids. This clone hybridized to polydisperse messages produced by human tracheo-bronchial and human colonic mucosae. The gene (proposed name MUC 4) from which cDNA is derived maps to chromosome 3.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Bronchi / physiology
  • Chromosomes, Human, Pair 3*
  • Cloning, Molecular
  • DNA / genetics
  • Humans
  • Molecular Sequence Data
  • Mucins / genetics*
  • Polymorphism, Restriction Fragment Length
  • RNA, Messenger / genetics
  • Trachea / physiology


  • Mucins
  • RNA, Messenger
  • DNA

Associated data

  • GENBANK/D00690
  • GENBANK/D00691
  • GENBANK/M62476
  • GENBANK/M62477
  • GENBANK/M62478
  • GENBANK/M62479
  • GENBANK/M62480
  • GENBANK/M62481
  • GENBANK/M62482
  • GENBANK/M64594