Phosphoester-transfer mechanism of an RNA-cleaving acidic deoxyribozyme revealed by radioactivity tracking and enzymatic digestion

Chem Commun (Camb). 2006 Jun 14;(22):2359-61. doi: 10.1039/b604682g. Epub 2006 May 9.

Abstract

A convenient method involving 32P-labeling of an RNA substrate at the cleavage site and subsequent enzymatic digestion of cleavage products via phosphatases reveals that pH4DZ1--an RNA-cleaving deoxyribozyme with optimal activity at pH 4--forms a 5'-cleavage fragment with 2',3'-cyclic phosphate group and a 3'-cleavage fragment with 5'-OH group.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Catalytic / chemistry*
  • Enzyme Activation
  • Hydrogen-Ion Concentration
  • Isotope Labeling
  • Organophosphates / chemistry*
  • Phosphorus Radioisotopes / chemistry
  • RNA / chemistry*

Substances

  • DNA, Catalytic
  • Organophosphates
  • Phosphorus Radioisotopes
  • RNA