Protein binding of cortisol by means of competitive adsorption: application to cortisol binding by serum of sixteen eutherian mammals

Comp Biochem Physiol A Comp Physiol. 1991;98(1):61-6. doi: 10.1016/0300-9629(91)90578-z.


1. Binding of 3H-cortisol by serum proteins by means of competitive adsorption was relatively high by serum of the gerbil, human, rabbit, sheep, tree shrew, hamster, rhesus monkey and horse. 2. A somewhat lower binding was observed by serum proteins of the baboon, cattle, dog, rat and cat. 3. Serum taken from either the mouse, guinea pig or pig gave very flat binding curves, specific binding not exceeding 5% of added 3H-cortisol. 4. It is concluded that the measurement of protein-binding of 3H-cortisol by means of competitive adsorption is a reliable method for serum of most eutherian species but is unsuited if serum of the mouse, guinea pig or pig is used.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Cortex Hormones / blood
  • Adsorption
  • Animals
  • Binding, Competitive / physiology
  • Hydrocortisone / blood*
  • Mammals / blood*
  • Protein Binding
  • Species Specificity
  • Transcortin / metabolism*
  • Tritium


  • Adrenal Cortex Hormones
  • Tritium
  • Transcortin
  • Hydrocortisone