Transfection assays with protoplasts containing integrated reporter genes

Methods Mol Biol. 2006;323:237-44. doi: 10.1385/1-59745-003-0:237.

Abstract

Transient expression assays with protoplasts that utilize stably integrated reporter genes along with transfected effector genes provide several advantages over assays in which both the reporter gene and effector gene(s) are transfected into protoplasts. A protocol for carrying out transient expression assays with Arabidopsis leaf mesophyll protoplasts containing single-copy integrated reporter genes is described.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / genetics*
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins
  • Biological Assay
  • Gene Expression Regulation, Plant
  • Genes, Plant
  • Genes, Reporter*
  • Genetic Techniques*
  • Glucuronidase
  • Plant Leaves
  • Promoter Regions, Genetic
  • Protoplasts / metabolism*
  • Transfection
  • Transformation, Genetic

Substances

  • Arabidopsis Proteins
  • Glucuronidase