Applications of chemical-inducible expression systems in functional genomics and biotechnology

Methods Mol Biol. 2006;323:329-42. doi: 10.1385/1-59745-003-0:329.

Abstract

The LexA-VP16-ER (XVE) system is an estrogen-receptor-based chemical-inducible system for use in plants. Protocols are outlined for the inducible overexpression of transgenes, inducible transgene excision, inducible gene silencing, regulated in vivo cDNA generation, inducible activation tagging, and the generation of conditional knockouts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics*
  • Biotechnology / methods*
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • Gene Expression Regulation, Plant
  • Gene Silencing
  • Genes, Plant
  • Genetic Techniques*
  • Models, Genetic
  • Phenotype
  • Plant Proteins / metabolism
  • Plants, Genetically Modified
  • RNA Interference
  • Receptors, Estrogen / genetics

Substances

  • DNA, Complementary
  • Plant Proteins
  • Receptors, Estrogen