Neutrophil cytoskeletal rearrangements during capillary sequestration in bacterial pneumonia in rats

Am J Respir Crit Care Med. 2006 Sep 15;174(6):689-98. doi: 10.1164/rccm.200502-276OC. Epub 2006 Jun 1.

Abstract

Rationale: Neutrophils accumulate in pulmonary capillaries during acute inflammation. Initial events in injury recognition and sequestration do not occur through selectin-mediated rolling. Cytoskeletal rearrangements, as assessed by submembrane F-actin rims, result in poorly deformable neutrophils that may not pass through capillaries.

Objective: To test the hypothesis that neutrophils sequestering during pneumonia contain F-actin rims and to determine the roles of CD11/CD18, L-selectin expression, and neutrophil-platelet adhesion in neutrophil sequestration.

Methods: Neutrophils were compared in blood obtained simultaneously from venous and arterial sites before and 4 h after instillation of Streptococcus pneumoniae or Escherichia coli in rats.

Measurements and main results: At 4 h of pneumonia, the number of neutrophils was greater in the venous blood entering the lungs than in the arterial blood leaving the lungs, indicating that neutrophil sequestration was occurring. More neutrophils entering the lungs contained F-actin rims than did neutrophils exiting, and the venous-arterial difference in F-actin-rimmed neutrophil counts completely accounted for sequestration. In E. coli pneumonia, in which neutrophil adhesion is mediated by CD11/CD18, CD18 blockade 15 min before blood samples were obtained did not prevent this sequestration of F-actin-rimmed neutrophils. Neutrophils expressing high or low levels of L-selectin or of neutrophils that bound platelets while circulating did not preferentially sequester.

Conclusions: Neutrophils with cytoskeletal rearrangements preferentially sequester within the lungs during pneumonia, and this sequestration is not due to CD11/CD18-mediated adhesion, L-selectin expression, or platelet adhesion to neutrophils, suggesting that cytoskeletal rearrangements result in sequestration of neutrophils.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • Animals
  • Biomarkers / metabolism
  • CD11 Antigens / metabolism
  • CD18 Antigens / metabolism
  • Capillaries / pathology
  • Cell Adhesion
  • Cytoskeleton / metabolism
  • Cytoskeleton / pathology*
  • Disease Models, Animal
  • Escherichia coli Infections / metabolism
  • Escherichia coli Infections / pathology
  • L-Selectin / metabolism
  • Lung / blood supply*
  • Lung / pathology
  • Neutrophils / metabolism
  • Neutrophils / pathology*
  • Pneumonia, Bacterial / metabolism
  • Pneumonia, Bacterial / pathology*
  • Pneumonia, Pneumococcal / metabolism
  • Pneumonia, Pneumococcal / pathology
  • Rats
  • Rats, Inbred Lew

Substances

  • Actins
  • Biomarkers
  • CD11 Antigens
  • CD18 Antigens
  • L-Selectin