Isolation and expression of a cDNA encoding Renilla reniformis luciferase
- PMID: 1674607
- PMCID: PMC51675
- DOI: 10.1073/pnas.88.10.4438
Isolation and expression of a cDNA encoding Renilla reniformis luciferase
Abstract
Renilla reniformis is an anthozoan coelenterate capable of exhibiting bioluminescence. Bioluminescence in Renilla results from the oxidation of coelenterate luciferin (coelenterazine) by luciferase [Renilla-luciferin:oxygen 2-oxidoreductase (decarboxylating), EC 1.13.12.5]. In vivo, the excited state luciferin-luciferase complex undergoes the process of nonradiative energy transfer to an accessory protein, green fluorescent protein, which results in green bioluminescence. In vitro, Renilla luciferase emits blue light in the absence of any green fluorescent protein. A Renilla cDNA library has been constructed in lambda gt11 and screened by plaque hybridization with two oligonucleotide probes. We report here the isolation and characterization of a luciferase cDNA and its gene product. The recombinant luciferase expressed in Escherichia coli is identical to native luciferase as determined by SDS/PAGE, immunoblot analysis, and bioluminescence emission characteristics.
Similar articles
-
Renilla reniformis bioluminescence: luciferase-catalyzed production of nonradiating excited states from luciferin analogues and elucidation of the excited state species involved in energy transfer to Renilla green fluorescent protein.Biochemistry. 1979 May 29;18(11):2204-10. doi: 10.1021/bi00578a011. Biochemistry. 1979. PMID: 36127 No abstract available.
-
Purification and properties of Renilla reniformis luciferase.Biochemistry. 1977 Jan 11;16(1):85-91. doi: 10.1021/bi00620a014. Biochemistry. 1977. PMID: 12797
-
A puzzling homology: a brittle star using a putative cnidarian-type luciferase for bioluminescence.Open Biol. 2017 Apr;7(4):160300. doi: 10.1098/rsob.160300. Open Biol. 2017. PMID: 28381628 Free PMC article.
-
Bioluminescence.Annu Rev Cell Dev Biol. 1998;14:197-230. doi: 10.1146/annurev.cellbio.14.1.197. Annu Rev Cell Dev Biol. 1998. PMID: 9891783 Review.
-
Coelenterazine-Type Bioluminescence-Induced Optical Probes for Sensing and Controlling Biological Processes.Int J Mol Sci. 2023 Mar 7;24(6):5074. doi: 10.3390/ijms24065074. Int J Mol Sci. 2023. PMID: 36982148 Free PMC article. Review.
Cited by
-
Uncovering the role of sorbitol in Renilla luciferase kinetics: Insights from spectroscopic and molecular dynamics studies.Biochem Biophys Rep. 2023 Dec 25;37:101617. doi: 10.1016/j.bbrep.2023.101617. eCollection 2024 Mar. Biochem Biophys Rep. 2023. PMID: 38371529 Free PMC article.
-
Mass spectrometry and split luciferase complementation assays reveal the MecA protein interactome of Streptococcus mutans.Microbiol Spectr. 2024 Feb 6;12(2):e0369123. doi: 10.1128/spectrum.03691-23. Epub 2024 Jan 17. Microbiol Spectr. 2024. PMID: 38230956 Free PMC article.
-
Catecholamine Involvement in the Bioluminescence Control of Two Species of Anthozoans.Life (Basel). 2023 Aug 23;13(9):1798. doi: 10.3390/life13091798. Life (Basel). 2023. PMID: 37763202 Free PMC article.
-
Creation of Artificial Luciferase 60s from Sequential Insights and Their Applications to Bioassays.Sensors (Basel). 2023 Jul 13;23(14):6376. doi: 10.3390/s23146376. Sensors (Basel). 2023. PMID: 37514669 Free PMC article.
-
Study of Protein Conformational Dynamics Using Hydrogen/Deuterium Exchange Mass Spectrometry.Methods Mol Biol. 2023;2652:293-318. doi: 10.1007/978-1-0716-3147-8_18. Methods Mol Biol. 2023. PMID: 37093484
References
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
