Primary structure of mouse tyrosine hydroxylase deduced from its cDNA

Biochem Biophys Res Commun. 1991 May 15;176(3):1610-6. doi: 10.1016/0006-291x(91)90472-j.

Abstract

The cDNAs for tyrosine hydroxylase were cloned from a mouse brain cDNA library by plaque hybridization. Since the longest cDNA clone lacked approximately 150 bp sequence of its N-terminal region, additional 5' region was obtained using polymerase chain reaction. Nucleotide sequence determination of cDNAs revealed that mouse tyrosine hydroxylase m-RNA encodes 498 amino acids with a calculated molecular mass of 55,990. The amino acid sequence of mouse tyrosine hydroxylase is highly homologous to rat (97%) and human (92%) enzymes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / enzymology
  • Cloning, Molecular
  • DNA / genetics*
  • Gene Library
  • Humans
  • Mice
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Plasmids
  • Polymerase Chain Reaction / methods
  • RNA, Ribosomal / genetics
  • RNA, Ribosomal / isolation & purification
  • Rats
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Tyrosine 3-Monooxygenase / genetics*

Substances

  • Oligonucleotide Probes
  • RNA, Ribosomal
  • DNA
  • Tyrosine 3-Monooxygenase

Associated data

  • GENBANK/M62480
  • GENBANK/M62481
  • GENBANK/M62482
  • GENBANK/M69190
  • GENBANK/M69191
  • GENBANK/M69192
  • GENBANK/M69193
  • GENBANK/M69194
  • GENBANK/M69200
  • GENBANK/X53944