A protocol for combined Photinus and Renilla luciferase quantification compatible with protein assays

Anal Biochem. 2006 Sep 1;356(1):94-9. doi: 10.1016/j.ab.2006.04.046. Epub 2006 May 12.


We established a quantitative reporter gene protocol, the P/Rluc assay system, allowing the sequential measurement of Photinus and Renilla luciferase activities from the same extract. Other than comparable commercial reporter assay systems and their noncommercial counterparts, the P/Rluc assay system was formulated under the aspect of full compatibility with standard methods for protein assays. This feature greatly expands the range of applications for assay systems quantifying the expression of multiple luciferase reporters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Enzyme Inhibitors / pharmacology
  • Fireflies / enzymology
  • Fireflies / genetics
  • Genes, Reporter
  • HeLa Cells
  • Humans
  • Luciferases, Firefly / analysis*
  • Luciferases, Firefly / antagonists & inhibitors
  • Luciferases, Firefly / genetics
  • Luciferases, Renilla / analysis*
  • Luciferases, Renilla / genetics
  • Luminescent Measurements / methods
  • Renilla / enzymology
  • Renilla / genetics


  • Enzyme Inhibitors
  • luciferase, Photinus
  • Luciferases, Renilla
  • Luciferases, Firefly