In vitro generation of tumor specific T cells that recognize a shared antigen of AML: molecular characterization of TCR genes

Leuk Res. 2007 Feb;31(2):195-202. doi: 10.1016/j.leukres.2006.04.007. Epub 2006 Jun 5.


The identification of immunologically relevant tumor antigens is hampered by the difficulty of generating tumor-specific cytotoxic T cells (CTL). We present data demonstrating in vitro induction of autologous acute myelogenous leukemia (AML)-specific CTL. The specific T cell receptor has been identified and cloned. The CTL demonstrated specific lysis to autologous tumor blasts, but not to autologous BLCL or the NK-sensitive target K562. The clone secreted GM-CSF, TNFa, and IFNg when stimulated with AML blasts from 3 of 11 patients or cell lines tested, but not with K562 or autologous B-LCL. These three AML samples share a single HLA Class I antigen, HLA-A24. The T cell receptor genes identified by molecular methods are Vbeta7.9-J2.3-Cbeta2 and Valpha17-J49-Calpha.

MeSH terms

  • Acute Disease
  • Amino Acid Sequence
  • Antigens, Neoplasm / immunology*
  • CD8-Positive T-Lymphocytes / immunology
  • Cell Line, Tumor
  • HLA Antigens / immunology*
  • Humans
  • Leukemia, Myeloid / genetics
  • Leukemia, Myeloid / immunology*
  • Molecular Sequence Data
  • Receptors, Antigen, T-Cell / genetics*
  • Receptors, Antigen, T-Cell / immunology
  • T-Lymphocytes, Cytotoxic / immunology*


  • Antigens, Neoplasm
  • HLA Antigens
  • Receptors, Antigen, T-Cell