The effect of salidroside on cell damage induced by glutamate and intracellular free calcium in PC12 cells

J Asian Nat Prod Res. 2006 Jan-Mar;8(1-2):159-65. doi: 10.1080/1028602042000325645.


Salidroside (Sald), was extracted from Rhodiola rosea L, a traditional Chinese medicine which has been used for long time for anti-aging, anti-cancer and anti-oxidative stress etc. In present experiment, salidroside could protect the PC12 cell against injuries caused by exposure of PC12 cells to 2 mmol/L glutamate for 15 min followed by incubation with serum-free medium for 24 h, which resembled the excitotoxin in vivo system. Furthermore, saldroside could decrease the [Ca2+]i of PC12 cells in Mg2+-free Hanks' solution and D-Hanks' solution but there was no effect on basal [Ca2+]i in Hanks' solution. The studies also indicated that salidroside inhibited the increases of [Ca2+]i induced by KCl and glutamate. In conclusion, salidroside may protect PC12 cell against glutamate excitotoxic damage through suppressing the excessive entry of Ca2+ and the release of the calcium stores.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Glands / cytology
  • Adrenal Glands / drug effects
  • Animals
  • Calcium / metabolism*
  • Calcium Signaling / drug effects*
  • Glucosides / chemistry
  • Glucosides / pharmacology*
  • Glutamic Acid / toxicity*
  • Intracellular Fluid / chemistry
  • Intracellular Fluid / metabolism
  • Molecular Structure
  • PC12 Cells
  • Phenols / chemistry
  • Phenols / pharmacology*
  • Potassium Chloride
  • Rats


  • Glucosides
  • Phenols
  • Glutamic Acid
  • Potassium Chloride
  • rhodioloside
  • Calcium