Isatin compounds as noncovalent SARS coronavirus 3C-like protease inhibitors

J Med Chem. 2006 Jun 15;49(12):3440-3. doi: 10.1021/jm0602357.


A series of isatin derivatives were synthesized and tested against SARS CoV 3C-like protease. Substitutions at the N-1 and C-5 positions were examined to elucidate the differences in substrate binding sites of the rhinovirus 3C protease and SARS CoV 3C-like protease. Compound 5f shows significant inhibition with an IC(50) of 0.37 microM. Further study showed that, unlike the irreversible covalent binding of isatin derivatives to human rhinovirus 3C protease, the compounds tested in this study are all noncovalent reversible inhibitors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Coronavirus 3C Proteases
  • Cysteine Endopeptidases / chemistry
  • Humans
  • Isatin / analogs & derivatives*
  • Isatin / chemical synthesis*
  • Isatin / chemistry
  • Models, Molecular
  • Severe acute respiratory syndrome-related coronavirus / enzymology*
  • Viral Proteins / antagonists & inhibitors*
  • Viral Proteins / chemistry


  • Viral Proteins
  • Isatin
  • Cysteine Endopeptidases
  • Coronavirus 3C Proteases