Gas chromatography-mass spectrometry

Methods Mol Biol. 2006;324:53-74. doi: 10.1385/1-59259-986-9:53.


Gas chromatography-mass spectrometry (GC-MS) has been used in hormone assays particularly for steroids in biological fluids. The combination of GC with MS exploits the high-resolving power of gas chromatography to separate closely related molecules, and the ability of the MS to provide precise data for identification and quantification of the separated substances. GC-MS is a very powerful technique for analysis with specificity of hormones in biological fluids. The general principles of GC-MS are described in this chapter along with some examples that illustrate specific applications of hormone analysis.

Publication types

  • Review

MeSH terms

  • Adrenal Cortex Hormones / analysis
  • Animals
  • Gas Chromatography-Mass Spectrometry / instrumentation
  • Gas Chromatography-Mass Spectrometry / methods*
  • Gonadal Steroid Hormones / analysis
  • Humans
  • Thyroid Hormones / analysis


  • Adrenal Cortex Hormones
  • Gonadal Steroid Hormones
  • Thyroid Hormones