Analysis of intrahepatic lymphocyte subsets in a transgenic mouse model of immune-mediated hepatocarcinogenesis

J Exp Clin Cancer Res. 2006 Mar;25(1):55-7.


Long-term, persistent liver cell injury increases the risk for hepatocellular carcinoma (HCC) development in chronic viral hepatitis. In support of this notion, we have developed a unique animal model of chronic immune-mediated liver disease that induces hepatocellular carcinogenesis using HBV transgenic mice; however, the intrahepatic inflammatory response was not precisely evaluated. The current study demonstrated that hepatitis B surface antigen (HBsAg)-specific cytotoxic T lymphocytes (CTLs) were detected at a frequency of 0.05% of CD8+ T lymphocytes in the liver, and that monocytes/macrophages were remarkably increased as the disease developed. These results suggest that a minimal number of intrahepatic virus-specific CTLs and the recruited monocytes/macrophages may contribute to the process of chronic liver inflammation.

MeSH terms

  • Animals
  • CD8-Positive T-Lymphocytes / metabolism
  • Carcinoma, Hepatocellular / pathology*
  • Disease Models, Animal
  • Hepatitis / genetics
  • Hepatitis, Viral, Human / genetics
  • Humans
  • Inflammation
  • Liver / pathology
  • Liver Neoplasms / pathology*
  • Macrophages / metabolism
  • Mice
  • Mice, Transgenic
  • Monocytes / metabolism
  • T-Lymphocytes, Cytotoxic / metabolism