Immunohistological evaluation of Ki-67, p63, CK19 and p53 expression in oral epithelial dysplasias

J Oral Pathol Med. 2006 Jul;35(6):369-75. doi: 10.1111/j.1600-0714.2006.00444.x.


Background: Oral squamous cell carcinoma develops through a multistep of genetic mutations, and the process can be morphologically recognized as oral epithelial dysplasia. To evaluate the hypothesis that distributional alterations of proliferating and stem cells may be a useful index to estimate the grading and development of epithelial dysplasia, we examined the distribution patterns according to stratified cell layers.

Methods: Sixty-two oral dysplasia cases according to the histological grades were immunohistologically examined and the nuclear expression of Ki-67 and p63 antigens was counted according to epithelial layers as labeling index.

Results: The Ki-67 labeling index in the basal and suprabasal layers and that of p63 in the basal layer showed a significant difference between low- and high-grade groups of epithelial dysplasia.

Conclusion: The architectural alteration of proliferating cell and stem cell distribution in the layers of epithelial dysplasias may provide useful information to evaluate the grading of oral epithelial dysplasias.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Analysis of Variance
  • Cell Proliferation
  • Epithelium / pathology
  • Female
  • Humans
  • Immunohistochemistry
  • Keratins / biosynthesis
  • Ki-67 Antigen / biosynthesis
  • Male
  • Membrane Proteins / biosynthesis
  • Middle Aged
  • Mouth Neoplasms / metabolism*
  • Mouth Neoplasms / pathology
  • Neoplasm Proteins / analysis
  • Neoplasm Proteins / biosynthesis*
  • Neoplastic Stem Cells / metabolism*
  • Precancerous Conditions / metabolism*
  • Precancerous Conditions / pathology
  • Tumor Suppressor Protein p53 / biosynthesis


  • CKAP4 protein, human
  • Ki-67 Antigen
  • Membrane Proteins
  • Neoplasm Proteins
  • Tumor Suppressor Protein p53
  • Keratins