Antioxidative and neuroprotective activities of extracts from the fruit hull of mangosteen (Garcinia mangostana Linn.)

Med Princ Pract. 2006;15(4):281-7. doi: 10.1159/000092991.


Objective: The aim of this study was to investigate the antioxidative and neuroprotective activities of various extracts from the fruit hull of mangosteen (Garcinia mangostana Linn., GM).

Materials and methods: Four extracts: water, 50% ethanol, 95% ethanol and ethyl acetate, were used. The antioxidative activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging assay at extract concentrations of 1, 10, 50 and 100 microg/ml. Based on the free radical scavenging activity of the extracts, two (water and 50% ethanol) were selected for their protective activity in NG108-15 neuroblastoma cells against H(2)O(2)-induced oxidative stress and for cell viability using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.

Results: All extracts exhibited antioxidative activity. The water and 50% ethanol extracts showed high free-radical scavenging activity with IC(50) values of 34.98 +/- 2.24 and 30.76 +/- 1.66 microg/ml, respectively. Both water and 50% ethanol extracts exhibited neuroprotective activity on NG108-15 cells. The highest activity was observed at the concentration of 50 microg/ml for both the water and 50% ethanol extracts. For cytotoxicity test, none of the extracts was toxic to the cells except at the high concentration of 100 microg/ml.

Conclusions: These results suggest that the water and 50% ethanol extracts from the fruit hull of GM may be potent neuroprotectants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Dose-Response Relationship, Drug
  • Free Radical Scavengers / pharmacology
  • Fruit
  • Garcinia mangostana*
  • Mice
  • Neuroprotective Agents / pharmacology*
  • Oxidative Stress / drug effects
  • Plant Extracts / pharmacology
  • Rats


  • Antioxidants
  • Free Radical Scavengers
  • Neuroprotective Agents
  • Plant Extracts