Human lung adenocarcinoma cell lines with different lung colonization potential (LCP), and a correlation between expression of sialosyl dimeric Le(x) (defined by MAb FH6) and LCP

Clin Exp Metastasis. May-Jun 1991;9(3):245-57. doi: 10.1007/BF01753728.

Abstract

Human lung adenocarcinoma sub-cell lines HAL-8, HAL-24 and HAL-33, showing different lung colonization potential (LCP), were established from human lung adenocarcinoma cell line KUM-LK-2 using repeated cloning with limiting dilution technique. Cell lines HAL-8 and -33 were characterized by high and low LCP, respectively, while HAL-24 did not give rise to lung colonies. The cell surface protein and carbohydrate profiles were determined by cell surface labeling (with lactoperoxidase-dependent 125I-iodination and galactose oxidase-NaB3H4, respectively) followed by SDS-gel electrophoresis. Various carbohydrate epitopes expressed at the cell surface were analysed by cytofluorometry using various monoclonal antibodies (MAbs) directed to Le(x), sialosyl-Le(x), sialosyl dimeric Le(x), T, Tn and sialosyl-Tn structures, which are often reported as being highly expressed in a variety of human cancers, particularly adenocarcinoma. Expression of sialosyl dimeric Le(x) (defined by MAb FH6) was high on HAL-8, moderate on HAL-33, and relatively low on HAL-24. In contrast, each of the three lines showed essentially equal expression (as determined by MAb reactivity) of sialosyl-Tn (defined by MAb TKH2), Le(x) (defined by MAb SH1), and Tn (defined by MAb 1E3). The cell lines showed extremely weak expression of T (defined by MAb HH8). LCP of HAL-8 and -33 was completely inhibited by sialidase treatment of cells. It is suggested that higher expression of sialosyl dimeric Le(x) (defined by MAb FH6) in HAL-8 cells may play an important role in higher potential of blood-borne lung colonization.

Publication types

  • Comparative Study

MeSH terms

  • Adenocarcinoma / metabolism
  • Adenocarcinoma / pathology*
  • Animals
  • Antibodies, Monoclonal / metabolism
  • Antigens, Surface / metabolism
  • Antigens, Tumor-Associated, Carbohydrate / metabolism
  • Carbohydrate Metabolism
  • Carbohydrate Sequence
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Glycosylation
  • Humans
  • Lewis X Antigen / metabolism*
  • Lung Neoplasms / metabolism
  • Lung Neoplasms / pathology*
  • Mice
  • Mice, Nude
  • Molecular Sequence Data
  • Neoplasm Metastasis / pathology
  • Neuraminidase / antagonists & inhibitors
  • Neuraminidase / pharmacology
  • Tumor Cells, Cultured

Substances

  • Antibodies, Monoclonal
  • Antigens, Surface
  • Antigens, Tumor-Associated, Carbohydrate
  • Lewis X Antigen
  • sialosyl dimeric Le(x) antigen
  • Neuraminidase