Requirements for effective IgE cross-linking on mast cells and basophils

Mol Nutr Food Res. 2006 Jul;50(7):620-4. doi: 10.1002/mnfr.200500272.


This article reviews the characteristics of high affinity IgE receptors (FcepsilonRI) and their role in the response to allergenic proteins. The requirements for successful cross-linking of FcepsilonRI on basophils and mast cells and subsequent degranulation by allergenic proteins will be explained in detail. Methods for in vitro analysis of allergen-induced mast cell and basophil degranulation will be described and issues/problems in applying these methods will be discussed. Finally, implications for manipulation of protein allergens will be discussed.

Publication types

  • Review

MeSH terms

  • Allergens / immunology
  • Basophils / immunology*
  • Cell Degranulation
  • Cross-Linking Reagents
  • Immunoglobulin E / chemistry*
  • Immunoglobulin E / immunology
  • Immunoglobulin E / metabolism
  • Immunoglobulin G / immunology
  • Mast Cells / immunology*
  • Proteins / immunology
  • Receptors, IgE / chemistry
  • Receptors, IgE / immunology
  • Receptors, IgE / metabolism


  • Allergens
  • Cross-Linking Reagents
  • Immunoglobulin G
  • Proteins
  • Receptors, IgE
  • Immunoglobulin E