EVI5 protein associates with the INCENP-aurora B kinase-survivin chromosomal passenger complex and is involved in the completion of cytokinesis

Exp Cell Res. 2006 Jul 15;312(12):2325-35. doi: 10.1016/j.yexcr.2006.03.032. Epub 2006 Apr 19.

Abstract

EVI5 has been shown to be a novel centrosomal protein in interphase cells. In this report, we demonstrate using immunofluorescence microscopy that EVI5 has a dynamic distribution during mitosis, being associated with the mitotic spindle through anaphase and remaining within the midzone and midbody until completion of cytokinesis. Knockdown of EVI5 using siRNA results in a multinucleate phenotype, which is consistent with an essential role for this protein in the completion of cytokinesis. The EVI5 protein also undergoes posttranslational modifications during the cell cycle, which involve phosphorylation in early mitosis and proteolytic cleavage during late mitosis and cytokinesis. Since the subcellular distribution of the EVI5 protein was similar to that characteristic of chromosomal passenger proteins during the terminal stages of cytokinesis, we used immunoprecipitation and GST pull-down approaches to demonstrate that EVI5 is associated with the aurora B kinase protein complex (INCENP, aurora B kinase and survivin). Together, these data provide evidence that EVI5 is an essential component of the protein machinery facilitating the final stages of cell septation at the end of mitosis.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Aurora Kinase B
  • Aurora Kinases
  • Blotting, Western
  • Cell Cycle / physiology
  • Cell Line
  • Cell Line, Tumor
  • Chromosomal Proteins, Non-Histone / metabolism*
  • Cytokinesis / physiology*
  • HeLa Cells
  • Humans
  • Immunoprecipitation
  • Inhibitor of Apoptosis Proteins
  • Microscopy, Confocal
  • Microtubule-Associated Proteins / metabolism*
  • Mitosis / physiology
  • Neoplasm Proteins / metabolism*
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Phosphoprotein Phosphatases / metabolism
  • Phosphorylation
  • Protein Binding
  • Protein Processing, Post-Translational / physiology
  • Protein-Serine-Threonine Kinases / metabolism*
  • RNA, Small Interfering / genetics
  • Spindle Apparatus / metabolism
  • Survivin

Substances

  • BIRC5 protein, human
  • Chromosomal Proteins, Non-Histone
  • EVI5 protein, human
  • INCENP protein, human
  • Inhibitor of Apoptosis Proteins
  • Microtubule-Associated Proteins
  • Neoplasm Proteins
  • Nuclear Proteins
  • RNA, Small Interfering
  • Survivin
  • AURKB protein, human
  • Aurora Kinase B
  • Aurora Kinases
  • Protein-Serine-Threonine Kinases
  • Phosphoprotein Phosphatases