Mismatched siRNAs downregulate mRNAs as a function of target site location

FEBS Lett. 2006 Jun 26;580(15):3694-8. doi: 10.1016/j.febslet.2006.05.056. Epub 2006 Jun 5.


In mammalian cells, RNA interference can be mediated by synthetic duplex RNAs, termed small interfering RNAs (siRNAs), which assist in cleaving completely complementary mRNA transcripts. MicroRNAs (miRNAs) are endogenous small RNAs that assist in translationally repressing mRNAs with regions of partial complementarity, but may also reduce transcript levels. Since miRNAs predominantly interact with the 3' UTRs of transcripts, we sought to ask if mismatched siRNAs mimicking miRNAs affect cognate mRNA levels as a function of target site location. We find that mismatched siRNAs targeting the 3' UTRs of two endogenous transcripts yield a greater reduction in mRNA levels than those targeting the coding region. Our findings demonstrate the importance of target site location within endogenous mRNAs for small RNAs associated with RNAi.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • ATP-Binding Cassette Transporters / genetics
  • ATP-Binding Cassette Transporters / metabolism
  • Base Pair Mismatch / genetics
  • Base Sequence
  • Binding Sites
  • Cell Line, Tumor
  • Down-Regulation*
  • Humans
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Small Interfering / genetics*
  • RNA, Small Interfering / metabolism*
  • beta Catenin / genetics
  • beta Catenin / metabolism


  • ATP-Binding Cassette Transporters
  • RNA, Messenger
  • RNA, Small Interfering
  • beta Catenin