Recurrent FBN1 mutation (R62C) in a Chinese family with isolated ectopia lentis

Am J Ophthalmol. 2006 Jun;141(6):1136-8. doi: 10.1016/j.ajo.2005.12.044.

Abstract

Purpose: To examine the fibrillin-1 (FBN1) gene for mutations in members of a Chinese family with isolated ectopia lentis.

Design: Clinically relevant laboratory investigation.

Methods: Family members underwent clinical examinations. Genomic DNA was extracted from leukocytes of peripheral blood from the available members and 100 controls for mutation analysis. The 65 exons of FBN1 were amplified by polymerase chain reaction and screened for mutations by a combination of denaturing high-performance liquid chromatography analysis and direct DNA sequencing.

Results: A mutation, c.184C-->T in exon 2 of FBN1, which results in substitution of arginine by cysteine at position 62 of the fibrillin-1 protein (p.R62C) in all affected family members but in none of the unaffected individuals.

Conclusions: A recurrent mutation of FBN1 gene resulted in an arginine-to-cysteine residue (p.R62C), is responsible for the patients with isolated ectopia lentis in a Chinese family.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Asian Continental Ancestry Group / genetics*
  • China / epidemiology
  • Chromatography, High Pressure Liquid
  • Ectopia Lentis / ethnology
  • Ectopia Lentis / genetics*
  • Exons / genetics
  • Female
  • Fibrillin-1
  • Fibrillins
  • Humans
  • Male
  • Microfilament Proteins / genetics*
  • Pedigree
  • Point Mutation*
  • Polymerase Chain Reaction
  • Recurrence

Substances

  • FBN1 protein, human
  • Fibrillin-1
  • Fibrillins
  • Microfilament Proteins