Efficient transformation of Legionella pneumophila by high-voltage electroporation

Microbiol Res. 2006;161(3):246-51. doi: 10.1016/j.micres.2005.09.001. Epub 2005 Nov 11.


A simple and reproducible method has been developed to transform Legionella pneumophila by electroporation. Effects of different conditions, including electric field strength, pulse length, DNA quality and cell density, were evaluated. Using our method, an efficiency of up to 6 x 10(7) transformants/microg DNA was obtained. This optimized transformation procedure should efficiently facilitate gene manipulations in L. pneumophila, such as plasmid transfer, transposon mutagenesis, library transformation for complementation cloning, etc.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Edetic Acid
  • Electroporation / methods*
  • Legionella pneumophila / genetics*
  • Plasmids
  • Transformation, Bacterial*


  • Edetic Acid