B cell-associated LFA-1 and T cell-associated ICAM-1 transiently cluster in the area of contact between interacting cells

Cell Immunol. 1991 Jan;132(1):45-55. doi: 10.1016/0008-8749(91)90005-v.


TNP-specific B cells interact with carrier-specific T hybridoma cells in an antigen-specific, MHC-restricted manner. The formation of T cell/B cell conjugates is time and temperature dependent and results in the formation of a broad area of close contact between the interacting cells. In order to determine which surface molecules on the two cells cluster at the interaction site. T cell/B cell conjugates were formalin-fixed at different times following conjugation and were stained with antibodies directed against cell surface molecules. Results of these studies indicate that the alpha- and beta-subunits of LFA-1 on B cells transiently cluster in the area of cell contact. Maximum clustering of LFA-1 occurs at 45 min, after which time LFA-1 redistributes on the surface of the B cells. Several other B cell-associated molecules (MHC Class II, ICAM-1, Ig, B220, J11D, or CD23) do not cluster at the interaction site at any time point. T cell-associated LFA-1 does not cluster with any specific pattern, but ICAM-1 does. Maximum clustering of ICAM-1 occurs 60 to 90 min after intercellular contact. After this time, ICAM-1 redistributes on the surface of the T cells. Although both the alpha- and beta-subunits of LFA-1 cluster at the interaction site on B cells, antibodies recognizing these subunits differ in their ability to affect conjugation. One antibody recognizing the alpha chain of LFA-1 (M17/4.2) inhibits T-cell/B cell conjugation, whereas another antibody that also recognizes the alpha chain-(G-48) enhances conjugation. In contrast, an antibody that recognizes LFA-1 beta (M18/2.a.8) has no effect. An antibody that recognizes ICAM-1 (YN/1.7), the ligand for LFA-1, inhibits conjugation. These data show that, during T cell/B cell interaction. LFA-1 on B cells and ICAM-1 on T cells transiently cluster with similar, albeit not identical, kinetics to the site of cell-cell contact.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes / cytology*
  • B-Lymphocytes / physiology
  • Cell Adhesion Molecules / physiology*
  • Cell Adhesion*
  • Cell Compartmentation
  • Cytochalasins / pharmacology
  • Fluorescent Antibody Technique
  • Immunologic Techniques
  • In Vitro Techniques
  • Intercellular Adhesion Molecule-1
  • Lymphocyte Cooperation
  • Lymphocyte Function-Associated Antigen-1 / physiology*
  • Mice
  • Mice, Inbred BALB C
  • T-Lymphocytes / cytology*
  • T-Lymphocytes / physiology


  • Cell Adhesion Molecules
  • Cytochalasins
  • Lymphocyte Function-Associated Antigen-1
  • Intercellular Adhesion Molecule-1