A Consensus Epitope Prediction Approach Identifies the Breadth of Murine T(CD8+)-cell Responses to Vaccinia Virus

Nat Biotechnol. 2006 Jul;24(7):817-9. doi: 10.1038/nbt1215. Epub 2006 Jun 11.

Abstract

The value of predictive algorithms for identifying CD8+ T (T(CD8+))-cell epitopes has not been adequately tested experimentally. Here we demonstrate that conventional bioinformatic methods predict the vast majority of T(CD8+)-cell epitopes derived from vaccinia virus WR strain (VACV-WR) in the H-2(b) mouse model. This approach reveals the breadth of T-cell responses to vaccinia, a widely studied murine viral infection model, and may provide a tool for developing comprehensive antigenic maps of any complex pathogen.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Animals
  • CD8-Positive T-Lymphocytes / immunology*
  • Computational Biology / methods*
  • Epitope Mapping / methods*
  • Epitopes, T-Lymphocyte / immunology*
  • Mice
  • Mice, Inbred C57BL
  • Vaccinia virus / immunology*

Substances

  • Epitopes, T-Lymphocyte