Urea hydrogen peroxide determination in whole blood using europium tetracycline probe

Lilia C Courrol; Maria H Bellini; Luiz V G Tarelho; Flávia R O Silva; Ronaldo D Mansano; Laércio Gomes; Nilson D Vieira Jr; Nestor Shor

doi:10.1016/j.ab.2006.05.015

Urea hydrogen peroxide determination in whole blood using europium tetracycline probe

Anal Biochem. 2006 Aug 1;355(1):140-4. doi: 10.1016/j.ab.2006.05.015. Epub 2006 Jun 2.

Authors

Lilia C Courrol¹, Maria H Bellini, Luiz V G Tarelho, Flávia R O Silva, Ronaldo D Mansano, Laércio Gomes, Nilson D Vieira Jr, Nestor Shor

Affiliation

¹ FATEC-SP/CEETEPS, São Paulo, SP, Brazil. lcourrol@fatecsp.br

PMID: 16769029
DOI: 10.1016/j.ab.2006.05.015

Abstract

We introduce the use of a lanthanide complex, tetracycline-europium, for the clinical diagnosis of urea hydrogen peroxide in human whole blood. The values obtained agree with the urea concentration variation verified in 49 patients, including 12 predialysis, 12 peritoneal, and 15 dialysis subjects, and 10 controls. This method is noninvasive and can help in the identification of renal and cardiac diseases.

Publication types

Comparative Study

MeSH terms

Biosensing Techniques / methods
Carbamide Peroxide
Drug Combinations
Fluorescence
Humans
Kidney Failure, Chronic / blood
Kidney Failure, Chronic / diagnosis
Kidney Failure, Chronic / therapy
Molecular Probes / chemistry*
Organometallic Compounds / chemistry*
Peroxides / blood*
Renal Dialysis
Reproducibility of Results
Sensitivity and Specificity
Tetracyclines / chemistry*
Urea / analogs & derivatives*
Urea / blood

Substances

Drug Combinations
Molecular Probes
Organometallic Compounds
Peroxides
Tetracyclines
europium(III) tetracycline
Carbamide Peroxide
Urea