Abstract
The binding of EF2 (elongation factor 2) and of ADP-ribosyl-EF 2 to rat liver ribosomes is inhibited by ricin. This result suggests that the native enzyme and its ADP-ribose derivative have the same or closely related binding sites on the ribosome. The inhibition by ricin of the binding of EF 2 to ribosomes is consistent with the previous observation that ricin affects EF 2-catalysed translocation during polypeptide chain elongation.
MeSH terms
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Adenosine Diphosphate / metabolism
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Animals
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In Vitro Techniques
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Liver / drug effects
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Molecular Weight
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NAD / metabolism
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Peptide Elongation Factors / antagonists & inhibitors*
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Plant Proteins / pharmacology*
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Protein Binding
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Protein Biosynthesis*
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Rats
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Ribose / metabolism
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Ribosomes / drug effects*
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Ribosomes / metabolism
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Ricin / pharmacology*
Substances
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Peptide Elongation Factors
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Plant Proteins
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NAD
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Adenosine Diphosphate
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Ribose
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Ricin