Quantification of glutamate, glutamine, and other metabolites in in vivo proton NMR spectroscopy

NMR Biomed. 1991 Apr;4(2):81-4. doi: 10.1002/nbm.1940040209.


A reliable quantification of in vivo 1H MRS spectra is hampered by extensive line overlap, by intensity distortions due to the water suppressing pulse sequence and complex modulation patterns of J-coupled spins, and by deviations between the experimental and model lineshapes in computer fitting programs. By correcting the experimental lineshape, using a suitable pulse sequence, and incorporating prior knowledge about the spin systems and the intensity distortions, a sufficient quantification is possible. If reliable T2 values are available absolute concentrations can be determined.

MeSH terms

  • Animals
  • Aspartic Acid / analogs & derivatives
  • Aspartic Acid / analysis
  • Cerebral Cortex / chemistry*
  • Glutamates / analysis*
  • Glutamic Acid
  • Glutamine / analysis*
  • Magnetic Resonance Spectroscopy / methods
  • Protons
  • Rats


  • Glutamates
  • Protons
  • Glutamine
  • Aspartic Acid
  • Glutamic Acid
  • N-acetylaspartate