Novel ELISA system for detection of N-ERC/mesothelin in the sera of mesothelioma patients

Cancer Sci. 2006 Sep;97(9):928-32. doi: 10.1111/j.1349-7006.2006.00246.x. Epub 2006 Jun 7.

Abstract

We have developed a novel enzyme-linked immunosorbent assay (ELISA) system for the detection of N-ERC/mesothelin in the serum of mesothelioma patients and have begun to examine its clinical usefulness. N-ERC/mesothelin is a 31-kDa protein that forms the N-terminal fragment of the full-length 71-kDa ERC/mesothelin protein, and is physiologically secreted into the blood of mesothelioma patients where it can be detected using our sandwich ELISA containing two antibodies (rabbit polyclonal anti-ERC/mesothelin antibody-282 and mouse monoclonal antibody 7E7). Our ELISA system has thus far detected much higher serum levels of N-ERC/mesothelin in mesothelioma patients than in healthy controls or patients with other lung or pleural diseases. In conclusion, N-ERC/mesothelin is a promising candidate tumor marker for mesothelioma.

MeSH terms

  • Animals
  • Antibodies
  • Antibodies, Monoclonal
  • Biomarkers, Tumor / blood*
  • Blotting, Western
  • COS Cells
  • Chlorocebus aethiops
  • Enzyme-Linked Immunosorbent Assay / methods*
  • GPI-Linked Proteins
  • HeLa Cells
  • Humans
  • Immunohistochemistry
  • Membrane Glycoproteins / blood*
  • Mesothelioma / blood*
  • Mesothelioma / diagnosis*
  • Mice
  • Rabbits

Substances

  • Antibodies
  • Antibodies, Monoclonal
  • Biomarkers, Tumor
  • GPI-Linked Proteins
  • Membrane Glycoproteins
  • mesothelin