Arginine-rich Intracellular Delivery Peptides Noncovalently Transport Protein Into Living Cells

Biochem Biophys Res Commun. 2006 Aug 4;346(3):758-67. doi: 10.1016/j.bbrc.2006.05.205. Epub 2006 Jun 9.


Plasma membranes of plant or animal cells are generally impermeable to peptides or proteins. Many basic peptides have previously been investigated and covalently cross-linked with cargoes for cellular internalization. In the current study, we demonstrate that arginine-rich intracellular delivery (AID) peptides are able to deliver fluorescent proteins or beta-galactosidase enzyme into animal and plant cells, as well as animal tissue. Cellular internalization and transdermal delivery of protein could be mediated by effective and nontoxic AID peptides in a neither fusion protein nor conjugation fashion. Therefore, noncovalent AID peptides may provide a useful strategy to have active proteins function in living cells and tissues in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arginine / metabolism*
  • Cell Line
  • Cell Line, Tumor
  • Cell Survival
  • Cells / cytology*
  • Cells / metabolism*
  • Cricetinae
  • Humans
  • Liver / cytology
  • Liver / metabolism
  • Mice
  • Onions / cytology
  • Onions / metabolism
  • Peptides / chemistry*
  • Peptides / genetics
  • Peptides / metabolism*
  • Peptides / toxicity
  • Plasmids / genetics
  • Protein Transport
  • Skin / cytology
  • Skin / metabolism


  • Peptides
  • Arginine