A Novel Nuclear-Localized Protein With Special Adenylate Kinase Properties From Caenorhabditis Elegans

FEBS Lett. 2006 Jul 10;580(16):3811-7. doi: 10.1016/j.febslet.2006.05.074. Epub 2006 Jun 12.

Abstract

The adrenal gland protein AD-004 like protein (ADLP) from Caenorhabditis elegans was cloned and expressed in Escherichia coli. Enzyme assays showed that ADLP has special adenylate kinase (AK) properties, with ATP and dATP as the preferred phosphate donors. In contrast to all other AK isoforms, AMP and dAMP were the preferred substrates of ADLP; CMP, TMP and shikimate acid were also good substrates. Subcellular localization studies showed a predominant nuclear localization for this protein, which is different from AK1-AK5, but similar to that of human AK6. These results suggest that ADLP is more likely a member of the AK6 family. Furthermore, RNAi experiments targeting ADLP were conducted and showed that RNAi treatment resulted in the suppression of worm growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylate Kinase / chemistry
  • Adenylate Kinase / isolation & purification
  • Adenylate Kinase / metabolism*
  • Amino Acid Sequence
  • Animals
  • Caenorhabditis elegans / cytology
  • Caenorhabditis elegans / enzymology*
  • Caenorhabditis elegans Proteins / chemistry
  • Caenorhabditis elegans Proteins / isolation & purification
  • Caenorhabditis elegans Proteins / metabolism*
  • Cell Nucleus / metabolism*
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Gene Expression
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Nuclear Proteins / metabolism
  • Phylogeny
  • Protein Transport
  • RNA Interference
  • Recombinant Fusion Proteins / metabolism
  • Sequence Alignment
  • Substrate Specificity

Substances

  • Caenorhabditis elegans Proteins
  • Nuclear Proteins
  • Recombinant Fusion Proteins
  • Adenylate Kinase
  • E02H1.6 protein, C elegans