B7-H3 and B7-H4 expression in non-small-cell lung cancer

Lung Cancer. 2006 Aug;53(2):143-51. doi: 10.1016/j.lungcan.2006.05.012. Epub 2006 Jun 19.


Inhibitory regulatory functions of B7-H3 and B7-H4 regarding T-cell activation have been reported recently. Little is known about the significance of human B7-H3 and B7-H4 expression in non-small-cell lung cancer (NSCLC), and we conducted the present study to address this issue in cell lines and tumor tissue from 70 patients. B7-H3 is over-expressed by all six NSCLC cell lines on both mRNA and protein level. B7-H4 is only transcripted by one cell line in which an alternatively spliced form was discovered. In tumor tissues, expression of B7-H3 and B7-H4 was found both on the cell membrane and in the cytoplasm. Thirty-seven percent of the specimens expressed B7-H3 and 43% B7-H4. The number of T infiltrating lymphoid cells (TILs) in the tumor tissues that expressed B7-H3 or B7-H4 was much lower than those who did not. There was a significant positive relation between the expression of B7-H3 and B7-H4, and high B7-H3 or B7-H4 expression was significantly more common in cases with lymph node metastasis. These observations suggest the contribution of B7-H3 and B7-H4 to immune dysfunction and tumor progression in NSCLC patients. B7-H3 and B7-H4 may be an important target for diagnosis and/or therapy of NSCLC.

MeSH terms

  • Adenocarcinoma / metabolism
  • Adenocarcinoma / pathology
  • Alternative Splicing
  • Antigens, CD
  • B7 Antigens
  • B7-1 Antigen / biosynthesis*
  • Carcinoma, Non-Small-Cell Lung / metabolism*
  • Carcinoma, Non-Small-Cell Lung / pathology*
  • Carcinoma, Squamous Cell / metabolism
  • Carcinoma, Squamous Cell / pathology
  • Cell Line, Tumor
  • Female
  • Flow Cytometry
  • Humans
  • Immunohistochemistry
  • Leukocyte Common Antigens / biosynthesis
  • Lung Neoplasms / metabolism*
  • Lung Neoplasms / pathology*
  • Lymphocytes, Tumor-Infiltrating / metabolism
  • Male
  • Middle Aged
  • RNA, Messenger / metabolism
  • Receptors, Immunologic
  • Reverse Transcriptase Polymerase Chain Reaction
  • V-Set Domain-Containing T-Cell Activation Inhibitor 1


  • Antigens, CD
  • B7 Antigens
  • B7-1 Antigen
  • CD276 protein, human
  • RNA, Messenger
  • Receptors, Immunologic
  • V-Set Domain-Containing T-Cell Activation Inhibitor 1
  • VTCN1 protein, human
  • Leukocyte Common Antigens