Amniotic membrane transplantation for persistent corneal ulcers and perforations in acute fungal keratitis

Cornea. 2006 Jun;25(5):564-72. doi: 10.1097/01.ico.0000227885.19124.6f.


Purpose: To report the therapeutic effect and complications of amniotic membrane transplantation (AMT) in acute fungal keratitis.

Methods: Diagnosis of fungal keratitis was confirmed by cultures in 23 eyes of 23 patients. The indications to perform AMT were to promote reepithelialization in non-healing ulcers or to prevent corneal perforation. Antifungal agents were administered throughout the whole course of hospitalization. Repeated cultures were performed immediately before AMT. The main outcome measurements were epithelial healing rate, necessity of therapeutic penetrating keratoplasty (TPK), and persistence of infection.

Results: During a mean follow-up time of 20.6 months +/- 23.22 (6-65 months) AMT was performed during the active phase of the keratitis (fungal culture was still positive) in 16 patients (69.6%), and during the inactive phase (fungal culture negative) in 7 patients (30.4%). Single-layer AMT was performed in 17 patients, and double-layer AMT was performed in 6 patients with corneal perforation and anterior chamber collapse. Complete epithelialization was observed in 12 patients (75%) in the active group and in 7 patients (100%) in the inactive group. Treatment failure requiring TPK was experienced in 4 patients (25%) in the active group. Persistent fungal keratitis was noted in 2 patients (8.7%) in that group. The final visual acuity improved in 17 cases, worsened in 2 cases, and remained unchanged in 4 cases. Twelve of the 23 eyes (52.2%) in this study preserved useful vision (20/400 and better) with or without subsequent surgeries.

Conclusion: AMT is effective in promoting epithelialization and preventing corneal perforations in acute fungal keratitis, and there is no risk of rejection. However, the risk of persistent or recurrent infection necessitates continued antifungal treatment and patient monitoring.

Publication types

  • Case Reports

MeSH terms

  • Acute Disease
  • Adult
  • Aged
  • Amnion / transplantation*
  • Antifungal Agents / therapeutic use
  • Combined Modality Therapy
  • Corneal Ulcer / microbiology*
  • Corneal Ulcer / physiopathology
  • Corneal Ulcer / surgery*
  • Epithelium, Corneal / physiology
  • Eye Infections, Fungal / microbiology
  • Eye Infections, Fungal / physiopathology
  • Eye Infections, Fungal / surgery*
  • Female
  • Follow-Up Studies
  • Fungi / isolation & purification
  • Humans
  • Keratoplasty, Penetrating
  • Male
  • Middle Aged
  • Mycoses / microbiology
  • Mycoses / surgery*
  • Rupture, Spontaneous / prevention & control
  • Treatment Failure
  • Treatment Outcome
  • Visual Acuity
  • Wound Healing / physiology


  • Antifungal Agents