A colorimetric assay for screening transketolase activity

Mark E B Smith; Ursula Kaulmann; John M Ward; Helen C Hailes

doi:10.1016/j.bmc.2006.06.008

A colorimetric assay for screening transketolase activity

Bioorg Med Chem. 2006 Oct 15;14(20):7062-5. doi: 10.1016/j.bmc.2006.06.008. Epub 2006 Jun 19.

Authors

Mark E B Smith¹, Ursula Kaulmann, John M Ward, Helen C Hailes

Affiliation

¹ Department of Chemistry, University College London, 20 Gordon Street, London WC1H OAJ, UK.

PMID: 16784864
DOI: 10.1016/j.bmc.2006.06.008

Abstract

A tetrazolium red-based colorimetric assay has been devised to screen for transketolase activity with a range of aldehyde acceptors. The colorimetric TK assay is able to detect >8% bioconversion using non-alpha-hydroxylated aldehydes as acceptor substrates and is significantly faster and more convenient to use than chromatographic procedures.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aldehydes / chemistry
Catalysis
Colorimetry / methods
Drug Evaluation, Preclinical / methods
Enzyme Activation
Ketones / chemical synthesis
Ketones / chemistry
Molecular Structure
Tetrazolium Salts / chemistry
Transketolase / chemistry*

Substances

Aldehydes
Ketones
Tetrazolium Salts
Transketolase