Involvement of Y-family DNA polymerases in mutagenesis caused by oxidized nucleotides in Escherichia coli

J Bacteriol. 2006 Jul;188(13):4992-5. doi: 10.1128/JB.00281-06.

Abstract

Escherichia coli DNA polymerase IV incorporated 2-hydroxy-dATP opposite template guanine or thymine and 8-hydroxy-dGTP exclusively opposite adenine in vitro. Mutator phenotypes in sod/fur strains were substantially diminished by deletion of dinB and/or umuDC. DNA polymerases IV and V may be involved in mutagenesis caused by incorporation of the oxidized deoxynucleoside triphosphates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Polymerase beta / physiology*
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Mutagenesis*
  • Nucleotides / metabolism*
  • Oxidation-Reduction

Substances

  • Nucleotides
  • DNA Polymerase beta