Detection of a Novel Protein Encoded by the Bottom-Component RNA of Cowpea Mosaic Virus, Using Antibodies Raised against a Synthetic Peptide

J Wellink; M Jaegle; R Goldbach

doi:10.1128/JVI.61.1.236-238.1987

Detection of a Novel Protein Encoded by the Bottom-Component RNA of Cowpea Mosaic Virus, Using Antibodies Raised against a Synthetic Peptide

J Virol. 1987 Jan;61(1):236-8. doi: 10.1128/JVI.61.1.236-238.1987.

Authors

J Wellink¹, M Jaegle, R Goldbach

Affiliation

¹ Department of Molecular Biology, Agricultural University, De Dreyen 11, 6703 BC Wageningen, The Netherlands.

Abstract

A peptide was synthesized that corresponded to a sequence in the cowpea mosaic virus bottom-component RNA-encoded 200-kilodalton polyprotein showing homology to the picornaviral 3C proteases. By injecting a rabbit with this peptide, antibodies were obtained that allowed the detection of a novel viral protein derived from the 200-kilodalton polyprotein. This protein, which had a size of 24 kilodaltons was found in both infected cowpea leaves and cowpea protoplasts.