A prospective trial is reported of an assessment of perineal cleansing as a means of reducing bacterial contamination of midstream urine samples. One hundred and ninety-two asymptomatic antenatal ambulatory patients were randomly allocated into cleansing and non-cleansing cohorts. Quantitative urine microscopy and culture showed no significant differences between the groups. It is concluded that perineal cleansing has no role to play in the collection of midstream urine specimens from ambulatory women.