Aromatic L-amino-acid decarboxylase (DOPA decarboxylase) gene expression in dopaminergic and serotoninergic cells of the rat brainstem

Neurosci Lett. 1991 Jun 24;127(2):203-6. doi: 10.1016/0304-3940(91)90794-t.

Abstract

In situ hybridization was performed in the rat brain to detect aromatic L-amino acid decarboxylase (AADC) mRNA using 35S-labeled oligonucleotide probes derived from rat kidney AADC cDNA. Results demonstrated AADC mRNA in areas containing dopaminergic and serotoninergic cell bodies. Combined immunohistochemistry for tyrosine- or tryptophan hydroxylase and in situ hybridization for AADC mRNA demonstrated the dopaminergic or serotoninergic nature of cells containing AADC mRNA. Tyrosine hydroxylase-positive mesencephalic neurons containing a very low or no AADC mRNA signal were also observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5-Hydroxytryptophan / metabolism
  • Amino Acid Sequence
  • Animals
  • Aromatic-L-Amino-Acid Decarboxylases / genetics*
  • Aromatic-L-Amino-Acid Decarboxylases / metabolism
  • Brain Stem / metabolism*
  • Gene Expression*
  • Levodopa / metabolism
  • Male
  • Molecular Sequence Data
  • Neurons / metabolism
  • RNA, Messenger / analysis
  • Rats
  • Rats, Inbred Strains
  • Tryptophan Hydroxylase / metabolism*
  • Tyrosine 3-Monooxygenase / metabolism*

Substances

  • RNA, Messenger
  • Levodopa
  • 5-Hydroxytryptophan
  • Tyrosine 3-Monooxygenase
  • Tryptophan Hydroxylase
  • Aromatic-L-Amino-Acid Decarboxylases