Three brain sodium channel alpha-subunit genes are clustered on the proximal segment of mouse chromosome 2

Genomics. 1991 Jul;10(3):666-72. doi: 10.1016/0888-7543(91)90450-s.

Abstract

We have used long-range physical mapping and restriction fragment length polymorphisms between two mouse species to determine the chromosomal organization and location of the genes encoding three distinct isoforms of the alpha-subunit of the brain sodium channel. Physical mapping by pulsed-field gel electrophoresis has established that Scn2a and Scn3a (genes encoding type II and type III sodium channel alpha-subunit isoforms) are physically linked and are separated by a maximum distance of 600 kb. The segregation of restriction fragment length variations in backcross progeny of a Mus musculus and Mus spretus mating indicates that Scn 1 a (gene encoding the type I sodium channel alpha subunit) and Scn2a are tightly linked and are separated by a distance of 0.7 cM. Linkage analysis in backcross and recombinant inbred (BXD and AKXD) strains of mice localized the three sodium channel genes to the proximal segment of mouse chromosome 2 and suggested the probable gene order centromere-Hc-Neb-Pmv7-Scn2a/Scn3a-Scn1a-Mpmv 14. These results indicate that the three isoforms of the brain sodium channel alpha-subunit are encoded by three distinct genes that share a common ancestral origin.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abnormalities, Multiple / genetics
  • Animals
  • Base Sequence
  • Brain Chemistry*
  • Chromosome Mapping
  • Crosses, Genetic
  • Genes
  • Genetic Linkage
  • Genetic Markers
  • Mice / genetics*
  • Molecular Sequence Data
  • Muridae / genetics
  • Muscular Dystrophy, Animal / genetics
  • Phylogeny
  • Polymorphism, Restriction Fragment Length
  • Recombination, Genetic
  • Sodium Channels / genetics*
  • Species Specificity

Substances

  • Genetic Markers
  • Sodium Channels