Partial characterization of the mouse alpha-sarcoglycan promoter and its responsiveness to MyoD

Biochim Biophys Acta. 2006 May;1759(5):240-6. doi: 10.1016/j.bbaexp.2006.04.007. Epub 2006 May 9.


The mouse alpha-sarcoglycan gene is expressed in muscle cells during differentiation, but its transcriptional regulation is not understood. We have characterized the promoter region of the mouse alpha-sarcoglycan gene. This region is composed of positive and negative regulatory elements that respond to the myogenic differentiation environment. Accordingly, MyoD transactivates the alpha-sarcoglycan full-length and the proximal promoter. Chromatin immunoprecipitation assays revealed that MyoD, TFIID, and TFIIB interact with the distal promoter in C2C12 myoblasts, a stage at which the alpha-SG promoter appears to drive basal activity. In myotubes, such factors are located concomitantly at the distal promoter and at a DNA region around the proximal promoter. In agreement with these results, TFIID and TFIIB co-immunoprecipitate with MyoD. We conclude that the alpha-SG promoter is activated by MyoD, which interacts with TFIID and TFIIB in a protein complex differentially located at the distal promoter and around the proximal promoter during myogenic cell differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Differentiation / genetics
  • Chromatin Immunoprecipitation
  • Gene Expression Regulation
  • Mice
  • Molecular Sequence Data
  • Muscle Development / genetics*
  • Muscle Fibers, Skeletal / metabolism
  • MyoD Protein / metabolism*
  • Myoblasts / cytology
  • Myoblasts / metabolism
  • Promoter Regions, Genetic*
  • Sarcoglycans / genetics*
  • Transcription Factor TFIIB / metabolism
  • Transcription Factor TFIID / metabolism
  • Transcriptional Activation*


  • MyoD Protein
  • MyoD1 myogenic differentiation protein
  • Sarcoglycans
  • Transcription Factor TFIIB
  • Transcription Factor TFIID