A rapid and sensitive method for determination of sorafenib in human plasma using a liquid chromatography/tandem mass spectrometry assay

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Feb 1;846(1-2):1-7. doi: 10.1016/j.jchromb.2006.06.005. Epub 2006 Jun 23.


A rapid, sensitive and specific method was developed and validated using LC/MS/MS for determination of sorafenib in human plasma. Sample preparation involved a single protein precipitation step by the addition of 0.1 mL of plasma with 0.5 mL acetonitrile. Analysis of the compounds of interest including the internal standard ([(2)H(3)(15)N] sorafenib) was achieved on a Waters X-Terra C(18) (150 mm x 2.1mm i.d., 3.5 microm) analytical column using a mobile phase consisting of acetonitrile/10 mM ammonium acetate (65:35, v/v) containing 0.1% formic acid and isocratic flow at 0.2 mL/min for 6 min. The analytes were monitored by tandem mass spectrometry with electrospray positive ionization. Linear calibration curves were generated over the range of 7.3-7260 ng/mL for the human plasma samples with values for the coefficient of determination of >0.96. The values for both within day and between day precision and accuracy were well within the generally accepted criteria for analytical methods (<15%).

Publication types

  • Research Support, N.I.H., Extramural
  • Validation Study

MeSH terms

  • Area Under Curve
  • Benzenesulfonates / blood*
  • Benzenesulfonates / pharmacokinetics
  • Calibration
  • Niacinamide / analogs & derivatives
  • Phenylurea Compounds
  • Protein Kinase Inhibitors / blood*
  • Protein Kinase Inhibitors / pharmacokinetics
  • Pyridines / blood*
  • Pyridines / pharmacokinetics
  • Reference Standards
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Sorafenib
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Tandem Mass Spectrometry / methods*


  • Benzenesulfonates
  • Phenylurea Compounds
  • Protein Kinase Inhibitors
  • Pyridines
  • Niacinamide
  • Sorafenib